Induced pluripotent stem cell line MUSIi006-A derived from hair follicle keratinocytes as a non-invasive somatic cell source


In this study, we used hair follicle keratinocytes for reprogramming. Collection of plucked hairs offers advantages over other somatic cells because no medical professional or operation room is required. Keratinocytes were isolated from plucked hairs of a 21-year-old healthy woman and characterized for the expression of cytokeratin 14 (CK14). Reprogramming of keratinocytes was performed using Sendai virus. Further characterization of the keratinocyte-derived iPSC line (designated as MUSIi006-A) confirmed that the cell line was pluripotent, free from Sendai viral genome and transgenes, and retained normal karyotype. Our method represents an easy, non-invasive and efficient approach for iPSC generation from hair samples. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Boonkaew B, Tapeng L, Netsrithong R, Vatanashevanopakorn C, Pattanapanyasat K, Wattanapanitch M
Journal Stem cell research
Publication Date 2018 Aug;31:79-82
PubMed 30031234
DOI 10.1016/j.scr.2018.07.007

Research Projects

Cell Lines