DF19-9-11T, iPS DF19-9-11T.H

General

Cell Line

hPSCreg name WAi001-A
Cite as:
WAi001-A (RRID:CVCL_K054)
Alternative name(s)
DF19-9-11T, iPS DF19-9-11T.H
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
MPIi005-A
(SF2.1, wt1)
WISCi003-A
(IPS(FORESKIN)-1)
WAi001-B
(DF19-9-7T, iPS-DF19-9-7T)
Last update 17th February 2024
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator WiCell Research Institute (WA)

External Databases

BioSamples SAMEA104492560
Cellosaurus CVCL_K054
Wikidata Q54898098

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclones

Donor Information

General Donor Information

Sex male
Age of donor (at collection) neonate

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes
Family history Not available
Is the medical history available upon request? No
Is clinical information available? No

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7783918

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. ATCC; https://www.lgcstandards-atcc.org/Products/All/CRL-2097.aspx#generalinformation
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? No
Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number
Is there other documentation provided to the donor for consenting purposes? No
Has the donor agreed to be re-contacted? Unknown
Has the donor been informed about how her/his data will be protected? No
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly prevent development of commercial products? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? No
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? Yes
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? No
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? No
Does consent permit access to medical records of the donor? No
Does consent permit access to any other source of information about the clinical treatment or health of the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved?
Approval number
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved?
Approval number
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Fibroblast from foreskin.
Synonyms
  • BJ fibroblast
Source cell origin
A retractable double-layered fold of skin and mucous membrane that covers the glans penis and protects the urinary meatus when the penis is not erect.
Synonyms
  • prepuce
  • preputium
  • penile prepuce
  • prepuce of male
  • preputium penis
  • foreskin
  • praeputium
  • prepucium
show more synonyms
Age of donor (at collection) neonate
Source cell line vendor ATCC

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
No

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
Unknown

Culture Conditions

Medium mTeSR™ 1

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
NANOG
Yes
SOX2
Yes
Score:
Marker Present Absent
mCpG
OCT4
Morphology pictures
Teratoma. All characterization data and associated methods reported in https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2758053/
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Mesoderm
Ont Id: UBERON_0000926
Ectoderm
Ont Id: UBERON_0000924

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 27

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY, available in publication
Karyotyping method: G-Banding

Other Genotyping (Cell Line)