Protocol to generate a microfluidic vessels-on-chip platform using human pluripotent stem cell-derived endothelial cells
Summary
Here, we present a protocol for producing a microfluidic vessel-on-chip platform using human pluripotent stem cell-derived endothelial cells (SC-ECs). We describe steps for manufacturing the 3D-printed chip, cell culturing to generate SC-ECs, hydrogel patterning, and the formation and cultivation of barrier-forming vessels. We then detail procedures for the retrieval of cells and media from the open microfluidic chip platform to enable multi-omics analysis. For complete details on the use and execution of this protocol, please refer to Marder et al.1. Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.
| Authors | Remmert C, Otgonbayar M, Perschel JA, Marder M, Meier M |
|---|---|
| Journal | STAR protocols |
| Publication Date | 2024 Sep 20;5(3):103300 |
| PubMed | 39244759 |
| PubMed Central | PMC11407103 |
| DOI | 10.1016/j.xpro.2024.103300 |