LRB02

General#

Cell Line

hPSCreg Name KUe002-A
Alternative name(s)
LRB02
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
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Provider

Generator University of Copenhagen (KU)
Derivation country Denmark

External Databases

Cellosaurus CVCL_B237

General Information

Publications
Is the cell line available in principle?
No

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

Ethics#

We are currently revising this section.

hESC Derivation#

Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
ZP removal technique Enzymatic
Cell isolation Immunosurgery
Cell seeding Isolated ICM

Culture Conditions#

Surface coating Gelatin
Feeder cells Mouse Embryonic Fibroblast Cell
Cellfinder Ont Id: EFO_0004040
Passage method Mechanically
O2 Concentration 7 %
CO2 Concentration 6 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 15 %
Supplements
L-Glutamine

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-1
No
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
GATA6
Yes
PAX6
Yes
ZFP42 (REX-1)
Yes
SOX7
Yes
STELLA
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY

Other Genotyping (Cell Line)