Generation of four iPSC lines from peripheral blood mononuclear cells (PBMCs) of an attention deficit hyperactivity disorder (ADHD) individual and a healthy sibling in an Australia-Caucasian family


Peripheral blood mononuclear cells were donated by a male teenager with clinically diagnosed attention deficit hyperactivity disorder (ADHD) under the Diagnostic and Statistical Manual of Mental Disorders IV criteria and his unaffected male sibling. Induced pluripotent stem cells were developed using integration-free Sendai Reprogramming factors containing OCT4, SOX2, KLF4, and c-MYC. All four iPSC lines displayed pluripotent cell morphology, pluripotency-associated factors at the DNA and protein level, alkaline phosphatase enzymatic activity and a male karyotype of 46, XY. All lines had capacity for in vitro differentiation into all the three germ layers. All were negative for Mycoplasma. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Tong J, Lee KM, Liu X, Nefzger CM, Vijayakumar P, Hawi Z, Pang KC, Parish CL, Polo JM, Bellgrove MA
Journal Stem cell research
Publication Date 2019 Jan;34:101353
PubMed 30622032
DOI 10.1016/j.scr.2018.11.014

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