RP25_UiPSC_mR5F_8
VHIRi003-A
General
Cell Line |
|
| hPSCreg name | VHIRi003-A |
| Cite as: | VHIRi003-A |
| Alternative name(s) |
RP25_UiPSC_mR5F_8
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 4th December 2024 |
| User feedback | |
Provider |
|
| Generator | Vall d'Hebron Institut de Recerca (VHIR) |
External Databases |
|
| BioSamples | SAMEA117479213 |
General Information |
|
| * Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
|
| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
External Databases (Donor) |
|
| BioSamples | SAMEA117479214 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Please provide contact information of the holder of the original Donor Information Sheet. | anna.duarri@vhir.org |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | Controlled Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Ethical approval Board-competent authority approval obtained by Comité de Ética 22 June 2022 and by the Catalan Authority for Stem Cell Research (Approval number: 10/2022) |
| Approval number | 10/2022 |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
|
| Source cell line name |
Urine Derived from same source line (potentially other lot and donor, see below):
|
| Source cell type |
The fluid that is excreted by the kidneys. It is stored in the bladder and discharged through the urethra.
Synonyms
|
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | mRNA |
| Is reprogramming vector detectable? |
No |
Vector free reprogramming |
|
| Type of used vector free reprogramming factor(s) |
mRNA
|
Other |
|
| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Medium |
mTeSR™ 1
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
Differentiation Potency
In vitro directed differentiation
| Marker | Expressed |
| AFP – alpha fetoprotein |
Unknown |
| SOX17 – SRY-box transcription factor 17 |
Unknown |
In vitro directed differentiation
| Marker | Expressed |
| GATA2 - GATA binding protein 2 |
Unknown |
| ACTN3 - actinin alpha 3 |
Unknown |
In vitro directed differentiation
| Marker | Expressed |
| TUBB3 – tubulin beta 3 class III |
Unknown |
| PAX6 – paired box 6 |
Unknown |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
Login to share your feedback, experiences or results with the research community.