Induced pluripotent stem cell (iPSC) line MLi-004A derived from a patient with recessive dystrophic epidermolysis bullosa (RDEB)


We have generated MLi004-A, a new induced pluripotent stem cell (iPSC) line derived from skin fibroblasts of a female patient with recessive dystrophic epidermolysis bullosa (RDEB). This iPSC line may be used as a model system for studies on skin integrity, the extracellular matrix and skin barrier function. The characterization of the MLi004-A cell line consisted of molecular karyotyping, next-generation sequencing of the COL7A1 alleles, pluripotency and differentiation potentials testing by immunofluorescence of associated markers in vitro. The MLi-004A line has been also tested for ability to differentiate into fibroblasts. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Ropret S, Jeriha J, Sorčan T, Rogar M, Zemljič Jokhadar Š, McGrath JA, Ilic D, Liovic M
Journal Stem cell research
Publication Date 2021 Aug;55:102463
PubMed 34284275
DOI 10.1016/j.scr.2021.102463

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