Generation of the human induced pluripotent stem cell line PUMCi005-A from a patient with Perrault syndrome
Summary
We generated PUMCi005-A, an induced pluripotent stem cell (iPSC) line, from dermal fibroblasts of a 32-year-old female Perrault syndrome patient with double heterozygous (794 G > A and 1181 G > A) mutations in the TWNK gene using Sendai viral delivery of OCT4, SOX2, KLF4, and c-MYC. The PUMCi005-A iPSC line carried the TWNK mutations, displayed typical iPSC morphology, expressed pluripotent stem cell markers, did not have integration of Sendai virus, and exhibited a normal karyotype and differentiation into three germ layers. Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Wang YB, Lv YF, Zhang Y, Wang W |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Mar;75:103318 |
| PubMed | 38295749 |
| DOI | 10.1016/j.scr.2024.103318 |