A collection of three integration-free iPSCs derived from old male and female healthy subjects


Here, we present the characterization of three iPSC lines derived from dermal fibroblasts of old healthy subjects. Fibroblasts were reprogrammed using Sendai viral vectors encoding OCT4, SOX2, KLF4 and c-MYC. The iPSCs expressed endogenous pluripotency markers, could generate the three germ layers (ectoderm, mesoderm and endoderm), maintained a stable karyotype, and were free from Sendai vectors and reprogramming factors. These integration-free iPSCs can serve for establishing control cell cultures in studies searching for phenotypes and mechanisms that could potentially be dysregulated in degenerative diseases. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Rodríguez-Traver E, Díaz-Guerra E, Rodríguez C, Arenas F, Orera M, Kulisevsky J, Moratalla R, Vicario C
Journal Stem cell research
Publication Date 2020 Jan;42:101663
PubMed 31794941
DOI 10.1016/j.scr.2019.101663

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