Generation of two induced pluripotent stem cell lines from a patient with compound heterozygous mutations in the USH2A gene
Summary
The human iPSC lines LEIi010-A and LEIi010-B were generated from the dermal fibroblasts of a patient with Usher syndrome using episomal plasmids containing OCT4, SOX2, KLF4, L-MYC, LIN28, mir302/367 microRNA and shRNA for p53. These iPSC lines carry compound heterozygous mutations (c.949C > A and c.1256G > T) in USH2A. LEIi010-A and LEIi010-B expressed pluripotent stem cell markers, had a normal karyotype and could be differentiated into endoderm, mesoderm and ectodermal lineages. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | McLenachan S, Wong EYM, Zhang X, Leith F, Moon SY, Zhang D, Chen SC, Thompson JA, McLaren T, Lamey T, De Roach JN, Atlas MD, Dilley RJ, Chen FK |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2019 Apr;36:101420 |
| PubMed | 30904819 |
| DOI | 10.1016/j.scr.2019.101420 |