Establishment of the NUMNi001-A human iPSC line from peripheral blood mononuclear cells of a healthy male donor
Summary
Using an integration-free episomal vector containing the reprogramming components hOCT3/4, hSox2/KLF4, hL-MYC/LIN28, mp53DD, and EBNA-1, peripheral blood mononuclear cells obtained from a healthy 42-year-old man were successfully reprogrammed into induced pluripotent stem cells (iPSCs). The reprogrammed iPSCs were cultured without feeder cells. They exhibited a normal karyotype, expressed pluripotency markers, and differentiated into cells representing all three germ layers. The NUMNi001-A line could serve as a control for investigating disease mechanisms. Copyright © 2025 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Tanaka A, Furuhashi K, Matsumoto J, Hattori K, Owaki A, Kawazoe T, Kato A, Onogi C, Watanabe Y, Koshi-Ito E, Kushima I, Ozaki N, Maruyama S |
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| Journal | Stem cell research |
| Publication Date | 2025 Jul 3;87:103765 |
| PubMed | 40633253 |
| DOI | 10.1016/j.scr.2025.103765 |