Royan H2

General#

Cell Line

hPSCreg Name RIe002-A
Alternative name(s)
Royan H2
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
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Provider

Generator Royan Institute (RI)
Derivation country Iran

External Databases

Cellosaurus CVCL_9383

General Information

Publications
Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics#

We are currently revising this section.

hESC Derivation#

Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
Expansion status 4
Stephenson 2007
ICM morphology B
Stephenson 2007
Trophectoderm morphology a
Stephenson 2007
ZP removal technique Chemical
Cell isolation Immunosurgery
Cell seeding Isolated ICM

Culture Conditions#

Surface coating Gelatin
Feeder cells
Yes
Passage method Enzymatically and Mechanically
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Fetal Calf Serum
Serum concentration: 20 %
Supplements
L-Glutamine

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Connexin43
Yes
SSEA-1
No
Alkaline Phosphatase
Yes
nodal
Yes
ZFP42 (REX-1)
Yes
LEFTY1
Yes
foxd3
Yes
CXCR4
Yes
EPHA2
Yes
FGF4
Yes
NANOG
Yes
PAX6
Yes
PROML1 (AC133)
Yes
SOX2
Yes
TAL1 (SCL/TAL)
Yes
TDGF1
Yes
THY1 (CD90)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)