Royan H2
RIe002-A
General
Cell Line |
|
| hPSCreg name | RIe002-A |
| Cite as: | RIe002-A (RRID:CVCL_9383) |
| Alternative name(s) |
Royan H2
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 28th July 2017 |
| User feedback | |
Provider |
|
| Generator |
Royan Institute (RI)
Contact:
Embryonic Stem Cell Biology |
| Derivation country | Iran |
External Databases |
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| Cellosaurus | CVCL_9383 |
| Wikidata | Q54950984 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes |
Donor Information
General Donor Information |
|
| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | |
Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent |
hESC Derivation
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
No |
| Expansion status |
4
|
| ICM morphology |
B
|
| Trophectoderm morphology |
a
|
| ZP removal technique | Chemical |
| Cell isolation | Immunosurgery |
| Cell seeding | Isolated ICM |
Culture Conditions
| Surface coating | Gelatin | ||||||
| Feeder cells |
Yes |
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| Passage method | Enzymatically and Mechanically | ||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Fetal Calf Serum Serum concentration: 20 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| Connexin43 |
Yes |
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| SSEA-1 |
No |
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| Alkaline Phosphatase |
Yes |
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| nodal |
Yes |
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| ZFP42 (REX-1) |
Yes |
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| LEFTY1 |
Yes |
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| foxd3 |
Yes |
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| CXCR4 |
Yes |
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| EPHA2 |
Yes |
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| FGF4 |
Yes |
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| NANOG |
Yes |
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| PAX6 |
Yes |
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| PROML1 (AC133) |
Yes |
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| SOX2 |
Yes |
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| TAL1 (SCL/TAL) |
Yes |
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| TDGF1 |
Yes |
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| THY1 (CD90) |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XX
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Other Genotyping (Cell Line) |
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