Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2
Rett syndrome (RTT) is a childhood neurodevelopmental disorder caused by mutations in MECP2. To study the molecular mechanisms underlying RTT, four sublines of H1 hESCs were generated, carrying a hemizygous knockout or mutant allele of MECP2. Exons 3 and 4 of MECP2 were targeted using the CRISPR/Cas9 nuclease system. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.
|Authors||Zeng R, Sidik H, Robinson KS, Zhong FL, Reversade B, Pouladi MA|
|Journal||Stem cell research|
|Publication Date||2019 Oct;40:101533|