Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2

Summary

Rett syndrome (RTT) is a childhood neurodevelopmental disorder caused by mutations in MECP2. To study the molecular mechanisms underlying RTT, four sublines of H1 hESCs were generated, carrying a hemizygous knockout or mutant allele of MECP2. Exons 3 and 4 of MECP2 were targeted using the CRISPR/Cas9 nuclease system. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Zeng R, Sidik H, Robinson KS, Zhong FL, Reversade B, Pouladi MA
Journal Stem cell research
Publication Date 2019 Aug 9;40:101533
PubMed 31450191
DOI 10.1016/j.scr.2019.101533

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