Generation of human erythroblast-derived iPSC line using episomal reprogramming system


Peripheral blood mononuclear cells were isolated and cultured to a pure pro-EBL population and reprogrammed using episomal plasmids. The pluripotency of transgene-free induced pluripotent stem cell (iPSC) line was verified by the expression of pluripotency-associated markers and by in vitro spontaneous differentiation towards the 3 germ layers. The iPSC line showed normal karyotype. Peripheral blood is a non-invasive easy accessible cell source and combined with EBL outgrowth in vitro, a routine process obtaining sufficient amount of homogenous cells can be obtained within a week. Using episomal delivery, pro-EBLs can be reprogrammed in a transgene-free, cost effective system. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Varga E, Hansen M, Wüst T, von Lindern M, van den Akker E
Journal Stem cell research
Publication Date 2017 Dec;25:30-33
PubMed 29040913
DOI 10.1016/j.scr.2017.10.001

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