UMGACBi001-A

General

Cell Line

hPSCreg name UMGACBi001-A
Cite as:
UMGACBi001-A (RRID:CVCL_D0NS)
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 5th October 2023
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Institute of Anatomy and Cell Biology (UMGACB)

External Databases

BioSamples SAMEA114401594
Cellosaurus CVCL_D0NS
Wikidata Q123033739

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex male
Ethnicity European

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA114401595

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? No
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. Universitätsmedizin Greifswald Klinik und Poliklinik für Innere Medizin A Frau Prof. Dr. Sylvia Stracke Ferdinand-Sauerbruch-Straße
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethics Commission of the University Medicine Greifswald
Approval number BB 166/19
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General

Source cell type
The fluid that is excreted by the kidneys. It is stored in the bladder and discharged through the urethra.
Synonyms
  • Urine
  • Urinary System, Urine
  • urine
  • URINE
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector
Is reprogramming vector detectable?
No

Vector free reprogramming

Type of used vector free reprogramming factor(s)
mRNA, miRNA

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Laminin
Feeder cells
No
Passage method
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SOX2
Yes
NANOG
Yes
OCT3/4
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
TBXT
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
OTX2
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46 XY

Other Genotyping (Cell Line)