KCL017_VHL4

General

Cell Line

hPSCreg name KCLe013-A
Cite as:
KCLe013-A (RRID:CVCL_A250)
Alternative name(s)
KCL017_VHL4
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 28th July 2017
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Provider

Generator King's College London (KCL)
Derivation country United Kingdom

External Databases

Cellosaurus CVCL_A250
NIHhESC NIHhESC-13-0217
Wikidata Q54899664

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is affected.
Synonyms
  • Hippel Lindau syndrome
  • von Hippel-Lindau syndrome

Ethics

Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number NIHhESC-13-0217
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? HFEA

hESC Derivation

Date of derivation 2009-08-23
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
Yes
Expansion status 5
Stephenson 2007
ICM morphology B
Stephenson 2007
Trophectoderm morphology b
Stephenson 2007
ZP removal technique Mechanical
Cell isolation Laser
Cell seeding Isolated ICM

Culture Conditions

Surface coating Cellstart
Feeder cells Human Foreskin Fibroblast
Cellfinder Ont Id: CELDA_000001419
O2 Concentration 5 %
CO2 Concentration 6 %
Medium Other medium:
Base medium: KOSR-XF
Main protein source: Knock-out serum replacement
Serum concentration: 15 %
Supplements
FGF 2

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)