KCL017_VHL4

General#

Cell Line

hPSCreg Name KCLe013-A
Alternative name(s)
KCL017_VHL4
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
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Provider

Generator King's College London (KCL)
Derivation country United Kingdom

External Databases

Cellosaurus CVCL_A250
NIHhESC NIHhESC-13-0217

General Information

Publications
Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
von Hippel-Lindau disease
The donor is affected.
Synonyms
  • von Hippel-Lindau syndrome
  • Von Hippel-Lindau syndrome (VHL)
  • Von Hippel-Lindau syndrome (disorder)
  • Hippel Lindau syndrome
show more synonyms

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2009-08-23
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
Yes
Expansion status 5
Stephenson 2007
ICM morphology B
Stephenson 2007
Trophectoderm morphology b
Stephenson 2007
ZP removal technique Mechanical
Cell isolation Laser
Cell seeding Isolated ICM

Culture Conditions#

Surface coating Cellstart
Feeder cells Human Foreskin Fibroblast
Cellfinder Ont Id: CELDA_000001419
O2 Concentration 5 %
CO2 Concentration 6 %
Medium Other medium:
Base medium: KOSR-XF
Main protein source: Knock-out serum replacement
Serum concentration: 15 %
Supplements
FGF 2

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Other Genotyping (Cell Line)