Generation of a CFTR knock-in reporter cell line (MHHi006-A-1) from a human induced pluripotent stem cell line


CFTR encodes for a chloride ion channel expressed primarily in secretory epithelia in the airways, intestine, liver and other tissues. Mutations in the CFTR gene have been identified in people suffering from Cystic Fibrosis. Here, we established a CFTR knock-in reporter cell line from a human iPSC line (MHHi006-A) using TALEN technology. The reporter enables the monitoring and optimization of the differentiation of pluripotent stem cells into CFTR expressing epithelia on a single cell level, as well as the enrichment of CFTR positive cells, which represent an excellent tool for Cystic Fibrosis disease modelling, drug screening and ultimately cellular therapies. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Engels L, Olmer R, de la Roche J, Göhring G, Ulrich S, Haller R, Martin U, Merkert S
Journal Stem cell research
Publication Date 2019 Oct;40:101542
PubMed 31473565
DOI 10.1016/j.scr.2019.101542

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