Generation of an induced pluripotent stem cell line (ZZUi020-A) from a patient with Parkinson's disease harboring the intermediate-length GGC repeat expansions in the NOTCH2NLC gene


Here, we describe the generation of an induced pluripotent stem cell (iPSC) line, from a female patient diagnosed with Parkinson's disease (PD). The patient carries a heterozygous intermediate-length GGC repeat expansions mutation in the NOTCH2NLC gene. Skin fibroblasts were reprogrammed using the non-integrating Sendai virus technology to deliver Klf4, OCT3/4, SOX2 and c-MYC factors. The generated iPSC line (ZZUi020-A) presented with expression of common pluripotency markers, showed potential of differentiating into derivatives of the three germ layers, and displayed a normal karyotype. The clone ZZUi020-A is presented thereafter, it can be used to study the mechanisms underlying NOTCH2NLC-PD pathogenesis. Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Fan Y, Liu F, Fan LY, Mao CY, Liu H, Zhang C, Yuan YP, Yang J, Wang YL, Liu YT, Shi CH, Xu YM
Journal Stem cell research
Publication Date 2021 Apr;52:102257
PubMed 33626493
DOI 10.1016/j.scr.2021.102257

Research Projects

Cell Lines