Generation of an induced pluripotent stem cell line (DANi-011A) from a Parkinson's disease patient with a LRRK2 p.G2019S mutation


We generated an induced pluripotent stem cell (iPSC) line from fibroblasts of a clinically diagnosed 70 year old female Parkinson's disease (PD) patient heterozygous for a pathogenic missense variant (p.G2019S; c. 6055 G > A) in the leucine-rich repeat kinase 2 (LRRK2) gene by using non-integrating Sendai viruses. The DANi-011A iPSC line has a normal karyotype and is free from Sendai viruses. The expression of pluripotent markers in the iPSC line was confirmed by immunofluorescent staining, and we confirmed its ability to differentiate into the three germ layers. The DANi-011A iPSC line can be used for modeling PD and as a drug-screening platform. Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Chen M, Maimaitili M, Buchholdt SH, Jensen UB, Febbraro F, Denham M
Journal Stem cell research
Publication Date 2020 May;45:101781
PubMed 32305865
DOI 10.1016/j.scr.2020.101781

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