Characterization of an induced pluripotent stem cell line (NCHi024-A) from a male infant with bicuspid aortic valve
Summary
Bicuspid aortic valve is one of the most common congenital heart defects which have only two leaflets instead of three. NCHi024-A is an induced pluripotent stem cell (iPSC) line derived from peripheral blood mononuclear cells (PBMCs) of a male infant with a bicuspid aortic valve. The line exhibits normal iPSC morphology, normal karyotype, and donor-matched identity. Markers of undifferentiated iPSC and differentiation into ectoderm, mesoderm, and endoderm were validated through immunofluorescence staining. NCHi024-A was tested negative for transgenes and mycoplasma contamination. This iPSC line can be differentiated into various types of cells and used for studying aortic valve disease. Copyright © 2025. Published by Elsevier B.V.
| Authors | Lee J, Hanley M, Ye S, Yu Y, Argall A, Zhao MT |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2025 Jun 3;87:103751 |
| PubMed | 40483903 |
| DOI | 10.1016/j.scr.2025.103751 |