Generation of a PDX1-EGFP reporter human induced pluripotent stem cell line, KSCBi005-A-3, using the CRISPR/Cas9 system
Summary
PDX1 plays a crucial role in the development and maintenance of β-cells and directly regulates pancreatic β-cell-specific transcription factors by binding to the insulin gene. Here, we introduced an EGFP reporter into the C-terminus of PDX1 in KSCBi005-A human induced pluripotent stem cells through homologous recombination using CRISPR/Cas9 nuclease. The cells had a normal karyotype, expressed several pluripotency markers, and maintained their differentiation potential. KSCBi005-A-3 cells can be used to monitor PDX1 expression in live cells during β-cell differentiation; the cell line has been registered at the National Stem Cell Bank, Korea National Institute of Health. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Lee Y, Choi HY, Kwon A, Park H, Park MH, Kim JW, Kim MJ, Kim YO, Kwak S, Koo SK |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2019 Dec;41:101632 |
| PubMed | 31683099 |
| DOI | 10.1016/j.scr.2019.101632 |