Generation of a PDX1-EGFP reporter human induced pluripotent stem cell line, KSCBi005-A-3, using the CRISPR/Cas9 system


PDX1 plays a crucial role in the development and maintenance of β-cells and directly regulates pancreatic β-cell-specific transcription factors by binding to the insulin gene. Here, we introduced an EGFP reporter into the C-terminus of PDX1 in KSCBi005-A human induced pluripotent stem cells through homologous recombination using CRISPR/Cas9 nuclease. The cells had a normal karyotype, expressed several pluripotency markers, and maintained their differentiation potential. KSCBi005-A-3 cells can be used to monitor PDX1 expression in live cells during β-cell differentiation; the cell line has been registered at the National Stem Cell Bank, Korea National Institute of Health. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Lee Y, Choi HY, Kwon A, Park H, Park MH, Kim JW, Kim MJ, Kim YO, Kwak S, Koo SK
Journal Stem cell research
Publication Date 2019 Dec;41:101632
PubMed 31683099
DOI 10.1016/j.scr.2019.101632

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