Generation of a PDX1-EGFP reporter human induced pluripotent stem cell line, KSCBi005-A-3, using the CRISPR/Cas9 system
PDX1 plays a crucial role in the development and maintenance of β-cells and directly regulates pancreatic β-cell-specific transcription factors by binding to the insulin gene. Here, we introduced an EGFP reporter into the C-terminus of PDX1 in KSCBi005-A human induced pluripotent stem cells through homologous recombination using CRISPR/Cas9 nuclease. The cells had a normal karyotype, expressed several pluripotency markers, and maintained their differentiation potential. KSCBi005-A-3 cells can be used to monitor PDX1 expression in live cells during β-cell differentiation; the cell line has been registered at the National Stem Cell Bank, Korea National Institute of Health. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.
|Authors||Lee Y, Choi HY, Kwon A, Park H, Park MH, Kim JW, Kim MJ, Kim YO, Kwak S, Koo SK|
|Journal||Stem cell research|
|Publication Date||2019 Dec;41:101632|