General

iPSC Line
hPSCreg name	AAUe003-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	AAUe003-A (RRID:CVCL_C742)
Alternative name(s)	CLS3
iPSC line type	Human embryonic stem cell (hESC)
Similar iPSC lines	No similar lines found.
Last update	28th July 2017
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Provider
Generator	Aalborg University (AAU) Contact: Laboratoriet for Stamcelleforskning
Derivation country	Denmark
External iPSC Databases
Cellosaurus	CVCL_C742
Wikidata	Q54813677
General iPSC Information
Publications	Lysdahl H et al. Derivation and characterization of four new human embryonic stem cell lines: the Danish experience. Reproductive biomedicine online. 2006 Jan;12(1):119-26. Zachar V et al. The effect of human embryonic stem cells (hESCs) long-term normoxic and hypoxic cultures on the maintenance of pluripotency. In vitro cellular & developmental biology. Animal. 2010 Apr;46(3-4):276-83.
* Is the cell line readily obtainable for third parties?	Yes

Donor Information

General Donor Information
Sex	female
Phenotype and Disease related information (Donor)
Diseases

Ethics

Alternatives to consent are available?	Yes
Alternatives to consent

hESC Derivation

Embryo stage	Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?	Yes
ZP removal technique	Enzymatic
Cell isolation	Immunosurgery
Cell seeding	Isolated ICM

iPSC Culture Conditions

Surface coating	Gelatin
Feeder cells	human foreskin fibroblasts Cellfinder Ont Id: CELDA_000001419
Passage method	Mechanically
CO2 Concentration	5 %
Medium	Other medium: Base medium: Knockout-DMEM Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements Supplement Amount Unit L-Glutamine bFGF

iPSC Characterisation

Analysis of undifferentiated iPSCs

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
SSEA-4	Yes
TRA 1-60	Yes
TRA 1-81	Yes
Telomerase Activity	Yes
FGF4	Yes
NANOG	Yes
POU5F1 (OCT-4)	Yes
SOX2	Yes

Differentiation Potency

Endoderm

Endoderm
Ont Id: UBERON_0000925

In vitro spontaneous differentiation

Mesoderm

Mesoderm
Ont Id: UBERON_0000926

In vitro spontaneous differentiation

Ectoderm

Ectoderm
Ont Id: UBERON_0000924

In vitro spontaneous differentiation

Genotyping

Karyotyping (iPSC Line)
Has the iPSC line karyotype been analysed?	Yes 46XX Passage number: 20
Other Genotyping (iPSC Line)