KMEB3

General#

Cell Line

hPSCreg Name SDUe003-A
Alternative name(s)
KMEB3
Cell line type Human embryonic stem cell (hESC)
Last update 22nd May 2019
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Provider

Generator University of Southern Denmark (SDU)
Derivation country Denmark

External Databases

Cellosaurus CVCL_A277

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics#

Alternatives to consent are available? Yes
Alternatives to consent

hESC Derivation#

Date of derivation 2005-12-12
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
Expansion status 2
Stephenson 2007
ICM morphology B
Stephenson 2007
Trophectoderm morphology b
Stephenson 2007
ZP removal technique Enzymatic
Cell isolation None
Cell seeding Embryo

Culture Conditions#

Surface coating Gelatin
Feeder cells mouse embryo fibroblast cell
Cellfinder Ont Id: EFO_0004040
Passage method Mechanically
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 15 %
Supplements
glutamax
hrbFGF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
TRA 1-81
Yes
nodal
Yes
SSEA-1
No
SSEA-4
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)