Generation of induced pluripotent stem cell line, NIMHi009-A, from PBMCs of an adult healthy male


Human induced pluripotent stem cells provide an exceptional platform for studying pathogenesis in vitro. We, therefore, have generated and characterized human induced pluripotent stem cell (iPSC) line NIMHi009-A derived from peripheral blood mononuclear cells (PBMCs) of healthy adult male control for an epileptic patient carrying voltage gated sodium channel mutation, using Sendai virus-based reprogramming. The generated iPSCs express pluripotency genes and can spontaneously differentiate into three germ layers. These cells display a normal karyotype and are free of mycoplasma. The iPSC line NIMHi009-A can be used as healthy control for modelling various diseases and screening for drugs. Copyright © 2024. Published by Elsevier B.V.

Authors Arunachal G, Milind Nimonkar M, Raghavendra K, Chetan GK, Mehta B, Markandeya YS
Journal Stem cell research
Publication Date 2024 Apr;76:103349
PubMed 38368738
DOI 10.1016/j.scr.2024.103349

Research Projects

Cell Lines