Generation of human iPSCs from human prostate cancer-associated fibroblasts IBPi002-A


A human induced pluripotent stem cell line was generated from cancer-associated fibroblasts of a 68-years old patient with diagnosed prostate adenocarcinoma (PCa). The fibroblast cell line was reprogrammed with Epi5™ Episomal iPSC Reprogramming Kit. Pluripotency of the derived transgene-free iPS cell line was confirmed both in vitro by detecting expression of factors of pluripotency on a single-cell level, and also in vivo using teratoma formation assay. This new iPS cell line may be used for differentiation into different prostate-specific cell types in differentiation studies. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Kahounová Z, Slabáková E, Binó L, Remšík J, Fedr R, Bouchal J, Kurfűrstová D, Vrtěl R, Študent V, Jurečková L, Porokh V, Hampl A, Souček K
Journal Stem cell research
Publication Date 2018 Dec;33:255-259
PubMed 30481741
DOI 10.1016/j.scr.2018.11.006

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