Generation of human iPSCs from human prostate cancer-associated fibroblasts IBPi002-A
A human induced pluripotent stem cell line was generated from cancer-associated fibroblasts of a 68-years old patient with diagnosed prostate adenocarcinoma (PCa). The fibroblast cell line was reprogrammed with Epi5™ Episomal iPSC Reprogramming Kit. Pluripotency of the derived transgene-free iPS cell line was confirmed both in vitro by detecting expression of factors of pluripotency on a single-cell level, and also in vivo using teratoma formation assay. This new iPS cell line may be used for differentiation into different prostate-specific cell types in differentiation studies. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.
|Kahounová Z, Slabáková E, Binó L, Remšík J, Fedr R, Bouchal J, Kurfűrstová D, Vrtěl R, Študent V, Jurečková L, Porokh V, Hampl A, Souček K
|Stem cell research