Xeno- and feeder-free derivation of two sex-discordant sibling lines of human embryonic stem cells


Human embryonic stem cells (hESCs) represent a virtually unlimited source of cells suitable for a variety of biomedical applications. However, a diminishing allogeneic background and undefined culture conditions are essential for developing robust and replicable protocols for differentiation experiments, disease modeling, and drug testing. Therefore, here we report the generation of the two sex-discordant sibling hESC lines, MUNIe008-A and MUNIe009-A, using the mechanical biopsy of vitrified-thawed embryos under xeno- and feeder-free conditions. The presented approach is applicable for deriving high-quality clinical-grade hESC lines for cell replacement therapies. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Souralova T, Holubcova Z, Kyjovska D, Hampl A, Koutna I
Journal Stem cell research
Publication Date 2021 Dec;57:102574
PubMed 34715503
DOI 10.1016/j.scr.2021.102574

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