SMUSHi003-A

General

Cell Line

hPSCreg name SMUSHi003-A
Cite as:
SMUSHi003-A (RRID:CVCL_D0LS)
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
BCRTi006-A
Donor's gene variants:
TRPC6
Donor diseases:
Focal Segmental Glomerulosclerosis
Last update 29th June 2023
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Southern Medical University Shenzhen hospital (SMUSH)

External Databases

BioSamples SAMEA113600920
Cellosaurus CVCL_D0LS
Wikidata Q123033535

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex female
Ethnicity Chinese

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • glomerulosclerosis, focal
  • glomerulosclerosis
  • glomerulosclerosis, focal segmental
  • glomerulonephritis, focal sclerosing
  • focal glomerulosclerosis
  • FGS
  • focal glomerular sclerosis
  • FSGS
  • FSGS - focal segmental glomerulosclerosis
  • FGS (focal glomerular sclerosis)
  • focal segmental glomerulosclerosis
show more synonyms
Family history yes
Is clinical information available? yes

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46,XX
Karyotyping method: G-Banding

External Databases (Donor)

BioSamples SAMEA113600921

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. Sourthern Medical University Shenzhen Hospital
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? Yes
Does consent prevent dissemination of genetic information? No
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? Yes
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? Yes
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? the committee of Southern Medical University Shenzhen hospital
Approval number NYSZYYEC20220046
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
General anatomical term which refers to nucleated and non-nucleated differentiated hemal cells.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium Essential 8™

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
TRA 1-60
Yes
NANOG
Yes
Marker Present Absent
mCpG
OCT4 X
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Morphology
AFP
SOX17
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Morphology
ACTA2
BRACHYURY
Ectoderm
Ont Id: UBERON_0000924
Morphology
TUBB3
SOX1

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)