Generation of a healthy heavy smoker patient-derived induced pluripotent stem cell line UHOMi007-A from peripheral blood mononuclear cells
Summary
Human pluripotent stem cells (hiPSC) represent a unique opportunity to model lung development and chronic bronchial diseases. We generated a hiPSC line from a highly characterized healthy heavy smoker male donor free from emphysema or tobacco related disease. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using integration-free Sendai virus. The cell line had normal karyotype, expressed pluripotency hallmarks, and differentiated into the three primary germ layers. The reported UHOMi007-A iPSC line may be used as a control to model lung development, study human chronic bronchial diseases and drug testing. Copyright © 2024 University Hospital Centre of Montpellier. Published by Elsevier B.V. All rights reserved.
| Authors | Ahmed E, Fieldès M, Bourguignon C, Mianné J, Petit A, Amel N, Foisset F, Bourdais C, Vachier I, Assou S, De Vos J, Bourdin A |
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| Journal | Stem cell research |
| Publication Date | 2024 Jun;77:103437 |
| PubMed | 38723411 |
| DOI | 10.1016/j.scr.2024.103437 |