ND29369-PARKIN/PRKN/PARK2-R275W-clone 4, ND29369-clone 4, ND29369-4, LCSBi004-B

General

Cell Line

hPSCreg Name
LCSBi004-B
Alternative name(s)
ND29369-PARKIN/PRKN/PARK2-R275W-clone 4, ND29369-clone 4, ND29369-4, LCSBi004-B
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 25th February 2022
Notes ND29369-4
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Provider

Generator Luxembourg Centre for Systems Biomedicine (LCSB)
Owner Luxembourg Centre for Systems Biomedicine (LCSB)
Distributors
Derivation country United States

External Databases

Cellosaurus CVCL_A8MA
Wikidata Q108820774
BioSamples SAMEA13166758
CLO CLO_0103766

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex female
Age of donor (at collection) 60-64
Ethnicity Hispanic/Latino, Dominican Republic

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Parkinson's Disease
family NINDS0667
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Parkinson Disease
  • Parkinson's disease
  • Parkinson's Disease
Disease associated phenotypes
  • Asymmetric onset
  • Bradykinesis
  • Gait difficulties
  • Postural Instability
  • Response to Anti-Parkinsonism Therapy tested and unresponsive
  • Resting Tremor
  • Rigidity
Non-disease associated phenotypes
  • former smoker, 24 years
Family history Family history of parkinsonism absent
Is the medical history available upon request? no
Is clinical information available? Coriell website

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
aCGH Probes (PASS/FAIL): Pass; SNP Probes (PASS/FAIL): Pass
Karyotyping method: Array CGH

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMN00805445

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: Coriell
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? No
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Unknown
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly prevent development of commercial products? Yes
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? Yes
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? No
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? Yes
Does consent prevent dissemination of genetic information? No
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? Yes
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? Yes
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? Yes
Does consent permit access to medical records of the donor? Yes
Please describe how access is provided: Coriell website
Does consent permit access to any other source of information about the clinical treatment or health of the donor? Yes
Contact data, institution, or website: Coriell website
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Samples were collected in accordance with the US Government guidelines and are subject to MTA issued by Coriell Institute for Medical Research NINDS Cell Repository. The iPSC reprogramming protocol was approved by the Committee on Human Research at the University of California, San Francisco.
Approval number
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Samples were collected in accordance with the US Government guidelines and are subject to MTA issued by Coriell Institute for Medical Research NINDS Cell Repository. The iPSC reprogramming protocol was approved by the Committee on Human Research at the University of California, San Francisco.
Approval number
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
Is there an MTA available for the cell line? Yes
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? CytoTune-iPS Sendai Reprogramming kit (ThermoFisher Scientific).
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General

Source cell line name ND29369
Source cell type
Fibroblast
Synonyms
  • fibroblast
  • Fibroblasts
  • Fibroblast
  • FIBROBLAST
show more synonyms
Source cell origin
skin fibroblast
Age of donor (at collection) 60-64
Source cell line vendor Coriell
Passage number reprogrammed 2

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR
Notes on reprogramming vector detection Scorecard
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Selection criteria for clones Oct3/4 and Tra-1-60 ICCC, morphology
Derived under xeno-free conditions
Yes
Derived under GMP?
Yes
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Accutase
CO2 Concentration 5 %
Medium mTeSR™ 1
Supplements
MTeSR supplement 1x %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
TRA 1-81
Yes
SSEA-4
Yes
SOX2
Yes
NANOG
Yes
Morphology pictures
ND29369-1 ND29369-4 TL.pdf
Transmitted light images
Self-renewal
Positive
Endoderm
Negative
Mesoderm
Negative
Ectoderm score
Unknown
Data analysis report
Differentiation Potency

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Not done
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
normal female 46 XX
Passage number: 16
Karyotyping method: G-Banding

Other Genotyping (Cell Line)