iTAF6-6

General#

Cell Line

hPSCreg Name IMGTi003-A
Alternative name(s)
iTAF6-6
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 6th September 2018
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Provider

Generator Research Institute of Medical Genetics, TNMRC (IMGT)
Derivation country Russia

External Databases

BioSamples SAMEA5044543

General Information

Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
neuropsychic and speech developmental delay (primary disease)
Disease associated phenotypes
  • neuropsychic and speech developmental delay, anxiety disorder, macrocephaly, microorchidism, multiple internal organs anomalies
Family history no

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46,XY,r(13)/45,XY,-13
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
Array CGH
arr[hg18] 3q12.2(101837302_101911026)×4 pat, 13q34(112153620_114077122)×1 dn

External Databases (Donor)

BioSamples SAMEA5044660

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Age of donor (at collection) 15-19
Collected in 2017
Passage number reprogrammed 2

Reprogramming method

Vector type Integrating
Vector Virus (Lentivirus)
Genes
Is the used vector excisable?
Unkown
Absence of reprogramming vector(s)?
Unkown
Reprogramming vectors silenced?
Unkown

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Gelatin
Feeder cells MEF
Passage method Mechanically
CO2 Concentration 5 %
Medium Other medium:
Base medium: DMEM/F12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
SSEA-4
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Protocol or reference
Mesoderm
Ont Id: UBERON_0000926
Protocol or reference
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Protocol or reference

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY,r(13)/45,XY,-13/46,XY,-13,+mar
Karyotyping method: G-Banding

Other Genotyping (Cell Line)