PHBI-ST-040, SCVI171

General

Cell Line

hPSCreg name SCVIi080-A
Cite as:
SCVIi080-A (RRID:CVCL_C7YI)
Alternative name(s)
PHBI-ST-040, SCVI171
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
SCVIi079-A
(SCVI167, PHBI-ST-036)
Donor diseases:
Idiopathic Pulmonary Hypertension
Last update 14th August 2023
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Provider

Generator Stanford Cardiovascular Institute (SCVI)

External Databases

Cellosaurus CVCL_C7YI
BioSamples SAMEA114071485
Wikidata Q123033420

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex female
Ethnicity Non-Hispanic

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • Idiopathic Pulmonary Arterial Hypertension
  • Idiopathic Pulmonary Hypertension
  • IPAH

External Databases (Donor)

BioSamples SAMEA114071486

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: Joseph C. Wu
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved?
Approval number
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • PBMC

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium Other medium:
Base medium: StemMACS™ iPS-Brew XF
Main protein source:
Serum concentration: %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
SOX2
Yes
POU5F1 (OCT-4)
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Protocol or reference
Mesoderm
Ont Id: UBERON_0000926
Marker Expressed
Brachyury
Yes
Protocol or reference
Ectoderm
Ont Id: UBERON_0000924
Protocol or reference

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal

Other Genotyping (Cell Line)