Generation and characterization of human induced pluripotent stem cells lines from four patients diagnosed with schizophrenia and one healthy control

Summary

Fibroblasts were isolated from skin biopsies of four patients diagnosed with schizophrenia and from one healthy control. Patient fibroblasts were transfected with five episomal, non-integrative reprogramming vectors to generate human induced pluripotent stem cells (iPSC). Reprogrammed iPSC showed consistent expression of several pluripotency markers, loss of expression of exogenous reprogramming vectors and ability to differentiate into all three germ layers. Additionally, iPSC maintained their normal karyotype during reprogramming. These generated cell lines can be used to study early neurodevelopmental and neuroinflammatory processes in schizophrenia in a patient-derived in vitro model. Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Stock R, Vogel S, Mau-Holzmann UA, Kriebel M, Wüst R, Fallgatter AJ, Volkmer H
Journal Stem cell research
Publication Date 2020 Aug 27;48:101961
PubMed 32911325
DOI 10.1016/j.scr.2020.101961

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