Establishment of a transgene-free iPS cell line (SDCHi007-A) from a young patient bearing a ATP1A2 mutation and suffering from Epilepsy
Summary
Epilepsy is a chronic neurological disease. Here we describe the generation of induced pluripotent stem cells (iPSCs) from a patient diagnosed as epilepsy caused by ATP1A2 gene mutation. Induced pluripotent stem cells (iPSCs) were developed using non-integrating episomal vectors containing OCT4, SOX2, KLF4, BCL-XL and C-MYC. The established iPSC line (SDCHi007-A) displayed pluripotent cell morphology, high expression levels of pluripotency markers, differentiation potential in vitro, normal karyotype, and remaining the original ATP1A2 gene mutation. Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Zhang H, Zhang T, Wang Y, Shi J, Meng Y, Zhang Q, Guo Q, Duan C |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Sep;79:103490 |
| PubMed | 39002250 |
| DOI | 10.1016/j.scr.2024.103490 |