Generation of a MSX1 knockout human embryonic stem cell line using CRISPR/Cas9 technology
The MSX1 gene encodes a transcriptional repressor and plays important roles in limb-pattern formation, craniofacial development, and odontogenesis during vertebrate embryogenesis. Previous studies demonstrated that human MSX1 mutations are associated with tooth agenesis, orofacial clefting, and nail dysplasia. Here, we generated a MSX1 knockout cell line from human embryonic stem cell (hESC) line (H9) by CRISPR/cas9-mediated gene targeting. This cell line may serve as a valuable in vitro cell model for MSX1 mutation-related diseases and help to gain more insight into the biological function of MSX1. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.
|Authors||Chiu W, Li A, Wang T, Li W, Zhang X|
|Journal||Stem cell research|
|Publication Date||2022 Apr;60:102729|