Induced pluripotent stem cell line from a mouse model of human azoospermia with a frameshift mutation TEX11_1260Ins(TT)
| Title | Induced pluripotent stem cell line from a mouse model of human azoospermia with a frameshift mutation TEX11_1260Ins(TT) |
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| Start date | 2020-06-01 |
| End date | 2020-11-30 |
| Sponsor | National Institutes of Health |
| Institution | University of Pittsburgh |
Project Description
Infertility is a common disease that impacts 15% of reproductive age couples worldwide and genetic causes are implicated in about half of those cases. Non-obstructive azoospermia is a severe for of male infertility that features spermatogenic failure resulting in no sperm in the ejaculate and severely reduces the chance to have biological children. We created a Tex11_1260Ins(TT) (1260GATATTGGTA) mutant mouse that models the TEX11_1258(TT) mutation identified from a patient with nonobstructive azoospermia. The Tex11_1260Ins(TT) iPSC cells displayed characteristics of pluripotent-like morphology, expressed pluripotent protein markers, showed normal karyotype, and were able to differentiate into tissues of the three germ layers.