Functional analysis and transcriptomic profiling of iPSC-derived macrophages and their application in modeling Mendelian disease
Summary
Our protocol-derived IPSDM are comparable with HMDM at phenotypic, functional, and transcriptomic levels. Tangier disease-IPSDM recapitulated hallmark features observed in HMDM and revealed novel inflammatory phenotypes. IPSDMs provide a powerful tool for study of macrophage-specific function in human genetic disorders as well as molecular studies of human macrophage activation and polarization. © 2015 American Heart Association, Inc.
| Authors | Zhang H, Xue C, Shah R, Bermingham K, Hinkle CC, Li W, Rodrigues A, Tabita-Martinez J, Millar JS, Cuchel M, Pashos EE, Liu Y, Yan R, Yang W, Gosai SJ, VanDorn D, Chou ST, Gregory BD, Morrisey EE, Li M, Rader DJ, Reilly MP |
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| Journal | Circulation research |
| Publication Date | 2015 Jun 19;117(1):17-28 |
| PubMed | 25904599 |
| PubMed Central | PMC4565503 |
| DOI | 10.1161/circresaha.117.305860 |