Generation of WTD, a control human iPSC line for genetic research
Summary
The establishment of well characterized control iPSC lines is essential for robust, reproducible research across laboratories. We used CRISPR/Cas9 to derive an isogeneic control line from a patient-derived iPSC line carrying a mutation in the NEFL gene (E396K). After correction of the E396K mutation, UCSFi003-A (WTD) exhibits multi-lineage differentiation potential, a normal karyotype, no large genomic abnormalities, and has consents for public distribution of cells and genomic data. Copyright © 2025 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Macklin BL, Runyon WV, Feliciano CM, Dierks PH, Kelly KR, Watry HL, Judge LM, Conklin BR |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2025 Nov 19;89:103872 |
| PubMed | 41330274 |
| DOI | 10.1016/j.scr.2025.103872 |