Protocol for the simultaneous detection of nuclear long non-coding RNAs and proteins in human iPSC-derived cardiomyocytes
Summary
Nuclear-enriched long non-coding RNAs (lncRNAs) can regulate gene expression by interacting with specific RNA-binding proteins (RBPs). Here, we present a protocol to directly image these lncRNAs at the single-molecule level together with their protein interactors in human cardiomyocytes. We describe steps to perform RNA-fluorescence in situ hybridisation (FISH) of a nuclear intron-retaining lncRNA, followed by sequential protein immunofluorescence (IF), enabling simultaneous visualisation of specific RNA and protein targets. We also detail procedures for probe design, cell seeding, and fixation. For complete details on the use and execution of this protocol, please refer to Buonaiuto et al.1. Copyright © 2026 The Author(s). Published by Elsevier Inc. All rights reserved.
| Authors | Buonaiuto G, Santini T, Ballarino M |
|---|---|
| Journal | STAR protocols |
| Publication Date | 2026 Jan 28;7(1):104351 |
| PubMed | 41615806 |
| PubMed Central | PMC12874303 |
| DOI | 10.1016/j.xpro.2026.104351 |