Generation and characterization of SOX17-specific EGFP expressing human induced pluripotent stem cell line, KSCBi017-A-4, using CRISPR/Cas9
Summary
We generated a human induced pluripotent stem cell (hiPSC) reporter line in which EGFP was inserted in-frame at the C-terminus of the endogenous SOX17 locus using CRISPR/Cas9-mediated homologous recombination. The targeted clone, KSCBi017-A-4, was isolated by puromycin selection and validated by PCR and Sanger sequencing. This SOX17-EGFP hiPSC line retains a normal karyotype and pluripotency and displays specific EGFP expression upon directed definitive endoderm differentiation. This reporter line provides a reliable tool for monitoring SOX17 expression during human endoderm specification. Copyright © 2026 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Yoo DH, Bayarsaikhan D, Lee J, Im YS, Bayarsaikhan G, Kang HA, Lee B, Kim YO |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2026 Feb 18;92:103943 |
| PubMed | 41747626 |
| DOI | 10.1016/j.scr.2026.103943 |