Generation of an ACTA2-EGFP reporter human induced pluripotent stem cell line, KITi001-C-41, using CRISPR/Cas9-mediated homologous recombination
Summary
Alpha-smooth muscle actin (α-SMA) is encoded by ACTA2 and is a key protein in the cellular contractile system of various mesodermal cell types, including hepatic stellate cells (HSCs), smooth muscle cells, and cardiomyocytes. α-SMA, which is a key protein in the development of hepatic fibrosis, is widely used as a reliable marker of HSC activation. Here, we generated an ACTA2-EGFP reporter human induced pluripotent stem cell line, KITi001-C-41, using a CRISPR/Cas9-based knock-in system. These reporter hiPSC lines can be used for lineage tracing of mesodermal cells and for screening of HSC activation factors. Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Jo S, Kim JW, Noh H, Kim H, Kim JH, Park HJ |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2021 Oct;56:102524 |
| PubMed | 34481189 |
| DOI | 10.1016/j.scr.2021.102524 |