Protocol for single-cell epigenetic profiling in human organoids and tumoroids with Epi-CyTOF
Summary
In this protocol, we present Epi-CyTOF, a cytometry by time of flight (CyTOF) approach for single-cell epigenome profiling in cortical organoids as a model system for human brain development. We describe steps for in silico panel design, antibody conjugation and testing, sample preparation and staining, and high-dimensional data analysis. Additionally, we provide recommendations for applying Epi-CyTOF to other model systems based on our experience with gastroesophageal tumoroids. For complete details on the use and execution of this protocol, please refer to Ditzer et al .1. Copyright © 2026 The Author(s). Published by Elsevier Inc. All rights reserved.
| Authors | Senoglu E, Mittné V, Dietz S, Eugster A, Thieme S, Baenke F, Albert M, Peitzsch C |
|---|---|
| Journal | STAR protocols |
| Publication Date | 2026 Jul 31;7(3):104695 |
| PubMed | 42541720 |
| PubMed Central | PMC13453457 |
| DOI | 10.1016/j.xpro.2026.104695 |