Microglia derived from human induced pluripotent stem cells are regulated by osteopontin, an endogenous extracellular matrix protein maintaining immune homeostasis

Summary

OPN induced a distinct early response profile in hiPSC-derived microglia, characterized by intermediate morphological remodeling, increased CD68 intensity, and reduced homeostatic gene expression, without overt pro-inflammatory gene expression. These findings support the role of OPN as a physiological priming signal in microglia and highlight hiPSC-derived microglia as a model for studying regulators of microglial modulation. Copyright © 2026 Olschewski, Koenig, Nerone, Gross, Šarić, Vay, Schroeter, Fink and Rueger.

Authors Olschewski DN, Koenig AM, Nerone HM, Gross E, Šarić T, Vay SU, Schroeter M, Fink GR, Rueger MA
Journal Frontiers in neuroscience
Publication Date 2026;20:1785992
PubMed 42422253
PubMed Central PMC13342188
DOI 10.3389/fnins.2026.1785992

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