Generation and characterization of an isogenic gene-corrected iPSC line CARIMi009-A-1 from a Hutchinson-Gilford Progeria Syndrome (HGPS) patient with a heterozygous G608G mutation in the LMNA gene

Summary

Hutchinson-Gilford Progeria Syndrome (HGPS) is an ultra-rare systemic laminopathy caused by a heterozygous point mutation in the LMNA gene encoding Lamin A/C (c.1824C > T, p.G608G). This synonymous mutation causes the production of a toxic form of Lamin A called Progerin. Integration of Progerin within the nuclear lamina disrupts cellular processes such as chromatin organization and gene transcription. Here we generated and characterized the induced isogenic pluripotency stem cell control line generated by correcting the c.1824C > T mutation. Used together with its parental line, this isogenic line excludes differences in genetic background while studying the pathophysiology of HGPS. Copyright © 2026. Published by Elsevier B.V.

Authors Lezzoche G, Ntotsia E, Seyen S, De Majo F, Olieslagers S, Mostert D, de Windt LJ
Journal Stem cell research
Publication Date 2026 Sep;95:104089
PubMed 42691791
DOI 10.1016/j.scr.2026.104089

Research Projects

Cell Lines