Typhi pathogenic potential
|Typhi pathogenic potential
|Marie Skłodowska-Curie Action (MSCA)
|Weizmann Institute of Science
Associated cell lines
The identification of the pathogenic potential, that is, the genetic elements conferring virulence and how they subvert the host’s defences, is fundamental. Here we aim at developing a pooled genetic screen using CRISPR interference to produce pathogen knocked-down libraries impaired in a curated subset of virulence factors of Salmonella Typhi. This pathogen is of particular interest because of the severity of its systemic infection combined with its human host-restriction, which left research lagging behind. Among other cellular systems, organoids obtained by WISi006-A line will be used as model for S. typhi infection.