Typhi pathogenic potential
|Title||Typhi pathogenic potential|
|Sponsor||Marie Skłodowska-Curie Action (MSCA)|
|Institution||Weizmann Institute of Science|
Associated cell lines
The identification of the pathogenic potential, that is, the genetic elements conferring virulence and how they subvert the host’s defences, is fundamental. Here we aim at developing a pooled genetic screen using CRISPR interference to produce pathogen knocked-down libraries impaired in a curated subset of virulence factors of Salmonella Typhi. This pathogen is of particular interest because of the severity of its systemic infection combined with its human host-restriction, which left research lagging behind. Among other cellular systems, organoids obtained by WISi006-A line will be used as model for S. typhi infection.