Non-invasive action potential recordings using printed electrolyte-gated polymer field-effect transistors
Summary
Scalable and high-throughput platforms to non-invasively record the Action Potentials (APs) of excitable cells are highly demanded to accelerate disease diagnosis and drug discovery. AP recordings are typically achieved with the invasive and low-throughput patch clamp technique. Non-invasive alternatives like planar multielectrode arrays cannot record APs without membrane poration, preventing accurate measurements of disease states and drug effects. Here, we disclose reliable and non-invasive recording of APs with patch clamp-like quality from human stem cell-derived cardiomyocytes using an inkjet-printed polymer semiconductor in an Electrolyte-Gated Field-Effect Transistor configuration. High sensitivity is proven by the detection of drug-induced pro-arrhythmic membrane potential oscillations as early/delayed afterdepolarizations. The higher throughput potential of this platform could significantly enhance disease modelling, drug screening, safety pharmacology and the study of abiotic/biotic interfaces. © 2025. The Author(s).
| Authors | Kyndiah A, Zemignani GZ, Ronchi C, Tullii G, Khudiakov A, Iachetta G, Chiodini S, Moreddu R, Viola FA, Schwartz PJ, Gomila G, De Angelis F, Sala L, Antognazza MR, Caironi M |
|---|---|
| Journal | Nature communications |
| Publication Date | 2025 Aug 31;16(1):8143 |
| PubMed | 40885735 |
| PubMed Central | PMC12398533 |
| DOI | 10.1038/s41467-025-63484-1 |