Establishment of the integration-free Class I and Class II 11 loci homozygous HLA iPSC line (MUSIi023-A) from peripheral blood mononuclear cells of a Thai donor

Summary

Pluripotent stem cell (PSC)-based technologies offer a promising new approach to generating functional cells or grafts for transplantation. To minimize the risk of immune rejection, induced pluripotent stem cells (iPSCs) that are homozygous for HLA Class I and Class II genes are a potential solution to reduce the risk of immune rejection in regenerative medicine applications. In this study, MUSIi023-A iPSC line was generated from peripheral blood mononuclear cells of a Thai donor with 11 loci homozygous HLA. This cell line is free of viral integration, exhibited pluripotent stem cell characteristics and able to differentiate to all embryonic lineages. Copyright © 2025 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Jiamvoraphong N, Nimsamer P, Wongsurawat T, Lorthongpanich C, Laowtammathron C, Issaragrisil S
Journal Stem cell research
Publication Date 2025 Oct;88:103840
PubMed 41033103
DOI 10.1016/j.scr.2025.103840

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