GENEA001, SIVF01
GENEAe001-A
General
Cell Line |
|
| hPSCreg name | GENEAe001-A |
| Cite as: | GENEAe001-A (RRID:CVCL_9010) |
| Alternative name(s) |
GENEA001, SIVF01
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 22nd May 2019 |
| User feedback | |
Provider |
|
| Generator |
Genea (GENEA)
Contact:
Biocells |
| Owner | Biocells |
| Distributors | |
| Derivation country | Australia |
External Databases |
|
| BioSamples | SAMEA104012400 |
| Cellosaurus | CVCL_9010 |
| NIHhESC | NIHhESC-16-0370 |
| Wikidata | Q54835562 |
General Information |
|
| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: allowed
|
Donor Information
General Donor Information |
|
| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
External Databases (Donor) |
|
| BioSamples | SAMEA104012399 |
Ethics
| Was the embryo established purely for research purposes? | No |
| Have both parents consented to the use of the embryo for ESC derivation? | Yes |
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Alternatives to consent are available? | Yes |
| Alternatives to consent | Consent not to be made public. Stage 2 consent will be sent separately. |
| Alternative consent approval number | NIHhESC-16-0370 |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | NHMRC |
| Approval number | Licence 309703 |
hESC Derivation
| Date of derivation | 2004-05-25 |
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
No |
| ZP removal technique | Enzymatic |
Culture Conditions
| Feeder cells |
Human Foreskin Fibroblasts Cellfinder Ont Id: CELDA_000001419 |
|||||||||
| Passage method | Mechanically | |||||||||
| Medium |
Other medium:
Base medium: DMEM/F-12
Main protein source: Fetal bovine serum Serum concentration: 20 % Supplements
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
|
||||
| SSEA-1 |
No |
|
||||
| SSEA-4 |
Yes |
|
||||
| TRA 1-60 |
Yes |
|
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XX
|
Other Genotyping (Cell Line) |
|
Login to share your feedback, experiences or results with the research community.