LMNA-D44-PE1-A43B
The cell line is not validated yet.
AUMCi015-A-1
General
Cell Line |
|
| hPSCreg name | AUMCi015-A-1 |
| Cite as: | AUMCi015-A-1 |
| Alternative name(s) |
LMNA-D44-PE1-A43B
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 19th March 2026 |
| User feedback | |
Provider |
|
| Generator | Amsterdam University Medical Centers (AUMC) |
| Owner | Amsterdam University Medical Centers (AUMC) |
| Distributors | |
| Derivation country | Netherlands |
External Databases |
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| BioSamples | SAMEA121979603 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: allowed
Additional restrictions:
The institute requires an MTA for academic parties and a license for all commercial parties. |
| Subclone of | |
Donor Information
General Donor Information |
|
| Sex | female |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
|
| Diseases | A disease was diagnosed.
|
| Family history | Brother died of DCM due to the same mutation |
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
| BioSamples | SAMEA120404906 |
Ethics
Also have a look at the ethics information for the parental line
AUMCi015-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | invitrogen |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
|
|
The source cell information can be found in the parental cell line
AUMCi015-A.
|
|
| Passage number reprogrammed | 1 |
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
Immunostaining, RT-PCR
|
| Notes on reprogramming vector detection | Parental line has been tested, genetic modified line has not been retested |
| Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
|
Other |
|
| Selection criteria for clones | Based on morphology |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
|||||
| SOX2 |
Yes |
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Morphology pictures
Morphology prime-edited line
Differentiation Potency
In vitro directed differentiation
| Marker | Expressed |
| LEFTY1 |
Yes |
| SOX17 |
Yes |
Protocol or reference
In vitro directed differentiation
| Marker | Expressed |
| TBX6 |
Yes |
| HAND1 |
Yes |
Protocol or reference
In vitro directed differentiation
| Marker | Expressed |
| ACTN1 |
Yes |
Protocol or reference
In vitro directed differentiation
| Marker | Expressed |
| SOX1 |
Yes |
| OTX2 |
Yes |
Protocol or reference
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
Genetic Modification
| Disease/phenotype related modifications |
|
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