General

Cell Line
hPSCreg name	BCHi020-A-4
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	BCHi020-A-4
Alternative name(s)	HNDS0163-01#B CC12(-/-)
Cell line type	Human induced pluripotent stem cell (hiPSC)
Similar lines	BCHi020-A-1 (HNDS0163-01#B CNC20(+/-)) Donor diseases: PTEN Hamartoma Tumor Syndrome BCHi020-A-2 (HNDS0163-01#B CC19(+/+)) Donor diseases: PTEN Hamartoma Tumor Syndrome BCHi020-A-3 (HNDS0163-01#B CC34(+/+)) Donor diseases: PTEN Hamartoma Tumor Syndrome BCHi020-A (HNDS0163-01#B) Donor diseases: PTEN Hamartoma Tumor Syndrome BCHi017-A-1 (HNDS0164-01#D CNC9(+/-)) Donor diseases: PTEN hamartoma tumor syndrome BCHi017-A-2 (HNDS0164-01#D CNC11(+/-)) Donor diseases: PTEN hamartoma tumor syndrome BCHi017-A-3 (HNDS0164-01#D CC2(+/+)) Donor diseases: PTEN hamartoma tumor syndrome BCHi017-A-4 (HNDS0164-01#D CC16(+/+)) Donor diseases: PTEN hamartoma tumor syndrome BCHi017-A-5 (HNDS0164-01#D CC3(-/-)) Donor diseases: PTEN hamartoma tumor syndrome BCHi017-A-6 (HNDS0164-01#D CC10(-/-)) Donor diseases: PTEN hamartoma tumor syndrome BCHi018-A-1 (HNDS0176-01#A CNC10(+/-)) Donor diseases: PTEN hamartoma tumor syndrome BCHi018-A-2 (HNDS0176-01#A CNC35(+/-)) Donor diseases: PTEN hamartoma tumor syndrome BCHi018-A-3 (HNDS0176-01#A CC3(+/+)) Donor diseases: PTEN hamartoma tumor syndrome BCHi018-A-4 (HNDS0176-01#A CC16(+/+)) Donor diseases: PTEN hamartoma tumor syndrome BCHi018-A-5 (HNDS0176-01#A CC38(-/-)) Donor diseases: PTEN hamartoma tumor syndrome BCHi018-A-6 (HNDS0176-01#A CC48(-/-)) Donor diseases: PTEN hamartoma tumor syndrome BCHi019-A-1 (HNDS0179-01#B CNC2(+/-)) Donor diseases: PTEN hamartoma tumor syndrome BCHi019-A-2 (HNDS0179-01#B CNC21(+/-)) Donor diseases: PTEN hamartoma tumor syndrome BCHi019-A-3 (HNDS0179-01#B CC13(+/+)) Donor diseases: PTEN hamartoma tumor syndrome BCHi019-A-4 (HNDS0179-01#B CC15(+/+)) Donor diseases: PTEN hamartoma tumor syndrome BCHi019-A-5 (HNDS0179-01#B CC26(-/-)) Donor diseases: PTEN hamartoma tumor syndrome BCHi014-A-1 (HNDS0141-01#B CNC22(+/+)) Donor diseases: PTEN hamartoma tumor syndrome BCHi014-A-2 (HNDS0141-01#B CNC35(+/+)) Donor diseases: PTEN hamartoma tumor syndrome BCHi014-A-3 (HNDS0141-01#B CC6(+/-)) Donor diseases: PTEN hamartoma tumor syndrome BCHi014-A-4 (HNDS0141-01#B CC43(+/-)) Donor diseases: PTEN hamartoma tumor syndrome BCHi014-A-5 (HNDS0141-01#B CC8(-/-)) Donor diseases: PTEN hamartoma tumor syndrome BCHi014-A-6 (HNDS0141-01#B CC23(-/-)) Donor diseases: PTEN hamartoma tumor syndrome BCHi016-A-2 (HNDS0151-01#B CNC43(+/-)) Donor diseases: PTEN hamartoma tumor syndrome BCHi016-A-3 (HNDS0151-01#B CC18(+/+)) Donor diseases: PTEN hamartoma tumor syndrome BCHi016-A-4 (HNDS0151-01#B CC23(+/+)) Donor diseases: PTEN hamartoma tumor syndrome BCHi013-A-1 (HNDS0143-01#A CNC21(+/-)) Donor diseases: PTEN hamartoma tumor syndrome BCHi013-A-2 (HNDS0143-01#A CNC24(+/-)) Donor diseases: PTEN hamartoma tumor syndrome BCHi013-A-3 (HNDS0143-01#A CC12(+/+)) Donor diseases: PTEN hamartoma tumor syndrome BCHi013-A-4 (HNDS0143-01#A CC28(+/+)) Donor diseases: PTEN hamartoma tumor syndrome BCHi015-A-1 (HNDS0138-01#B CNC24(+/-)) Donor diseases: PTEN hamartoma tumor syndrome BCHi015-A-2 (HNDS0138-01#B CNC42(+/-)) Donor diseases: PTEN hamartoma tumor syndrome BCHi015-A-3 (HNDS0138-01#B CC38(+/+)) Donor diseases: PTEN hamartoma tumor syndrome BCHi015-A-4 (HNDS0138-01#B CC51(+/+)) Donor diseases: PTEN hamartoma tumor syndrome BCHi015-A-5 (HNDS0138-01#B CC6(-/-)) Donor diseases: PTEN hamartoma tumor syndrome BCHi015-A-6 (HNDS0138-01#B CC11(-/-)) Donor diseases: PTEN hamartoma tumor syndrome BCHi016-A-1 (HNDS0151-01#B CNC20(+/-)) Donor diseases: PTEN hamartoma tumor syndrome BCHi017-A (HNDS0164-01#D) Donor diseases: PTEN hamartoma tumor syndrome BCHi018-A (HNDS0176-01#A) Donor diseases: PTEN hamartoma tumor syndrome BCHi019-A (HNDS0179-01#B) Donor diseases: PTEN hamartoma tumor syndrome BCHi014-A (HNDS141-01#B) Donor diseases: PTEN hamartoma tumor syndrome BCHi013-A (HNDS0143-01#A) Donor diseases: PTEN hamartoma tumor syndrome CBIGi001-A-2 (PINK1-KO, PINK1-KO/AIW002-02) CBIGi001-A-3 (PINK1-KO/PRKN-KO/AIW002-02, PINK1-KO/PRKN-KO) BCHi015-A (HNDS0138-01#B) Donor diseases: PTEN hamartoma tumor syndrome BCHi016-A (HNDS0151-01#B) Donor diseases: PTEN hamartoma tumor syndrome
Last update	24th February 2024
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Provider
Generator	Children's Hospital (BCH) Contact: Children's Hospital (BCH)
External Databases
BioSamples	SAMEA115345248
General Information
* Is the cell line readily obtainable for third parties?	Yes Research use: allowed Clinical use: not allowed Commercial use: not allowed
Subclone of	BCHi020-A

Donor Information

General Donor Information
Sex	male
Ethnicity	White; American
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. PTEN Hamartoma Tumor Syndrome The donor is affected. Synonyms PHTS PTEN Hamartoma Tumor Syndrome
Karyotyping (Donor)
Has the donor karyotype been analysed?	No
External Databases (Donor)
BioSamples	SAMEA115345244

Ethics

Also have a look at the ethics information for the parental line BCHi020-A .

Is there an MTA available for the cell line?	No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General
The source cell information can be found in the parental cell line BCHi020-A.
Reprogramming method
Vector type	Non-integrating
Vector	Sendai virus
Vector free reprogramming
Other
Derived under xeno-free conditions	Unknown
Derived under GMP?	Unknown
Available as clinical grade?	Unknown

Culture Conditions

Surface coating	Matrigel/Geltrex
Feeder cells	No
Medium	Other medium: Base medium: Stemflex Main protein source: Bovine Serum Albumin (BSA) Serum concentration: 1 %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?	Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?	No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?	Yes

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line BCHi020-A .

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46, XY; no clonal abnormalities Karyotyping method: G-Banding
Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications

PTEN hamartoma tumor syndrome Synonyms PHTS PTEN hamartoma tumor syndrome Genetic modifications PTEN (target) Type of modification Isogenic modification Chromosome location cytoband location: 10q23 Nucleotide sequence variant in HGVS format NM_000314.8: c.389 G>A, p.R130Q Is the modification homozygous or heterozygous? Homozygous How has the target locus been modified? Changed