BCHi020-A-4

HNDS0163-01#B CC12(-/-)

General

Cell Line

hPSCreg name BCHi020-A-4
Cite as:
BCHi020-A-4
Alternative name(s)
HNDS0163-01#B CC12(-/-)
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
BCHi020-A-1
(HNDS0163-01#B CNC20(+/-))
Donor diseases:
PTEN Hamartoma Tumor Syndrome
BCHi020-A-2
(HNDS0163-01#B CC19(+/+))
Donor diseases:
PTEN Hamartoma Tumor Syndrome
BCHi020-A-3
(HNDS0163-01#B CC34(+/+))
Donor diseases:
PTEN Hamartoma Tumor Syndrome
BCHi020-A
(HNDS0163-01#B)
Donor diseases:
PTEN Hamartoma Tumor Syndrome
BCHi017-A-1
(HNDS0164-01#D CNC9(+/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi017-A-2
(HNDS0164-01#D CNC11(+/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi017-A-3
(HNDS0164-01#D CC2(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi017-A-4
(HNDS0164-01#D CC16(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi017-A-5
(HNDS0164-01#D CC3(-/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi017-A-6
(HNDS0164-01#D CC10(-/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi018-A-1
(HNDS0176-01#A CNC10(+/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi018-A-2
(HNDS0176-01#A CNC35(+/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi018-A-3
(HNDS0176-01#A CC3(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi018-A-4
(HNDS0176-01#A CC16(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi018-A-5
(HNDS0176-01#A CC38(-/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi018-A-6
(HNDS0176-01#A CC48(-/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi019-A-1
(HNDS0179-01#B CNC2(+/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi019-A-2
(HNDS0179-01#B CNC21(+/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi019-A-3
(HNDS0179-01#B CC13(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi019-A-4
(HNDS0179-01#B CC15(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi019-A-5
(HNDS0179-01#B CC26(-/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi014-A-1
(HNDS0141-01#B CNC22(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi014-A-2
(HNDS0141-01#B CNC35(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi014-A-3
(HNDS0141-01#B CC6(+/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi014-A-4
(HNDS0141-01#B CC43(+/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi014-A-5
(HNDS0141-01#B CC8(-/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi014-A-6
(HNDS0141-01#B CC23(-/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi016-A-2
(HNDS0151-01#B CNC43(+/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi016-A-3
(HNDS0151-01#B CC18(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi016-A-4
(HNDS0151-01#B CC23(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi013-A-1
(HNDS0143-01#A CNC21(+/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi013-A-2
(HNDS0143-01#A CNC24(+/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi013-A-3
(HNDS0143-01#A CC12(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi013-A-4
(HNDS0143-01#A CC28(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi015-A-1
(HNDS0138-01#B CNC24(+/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi015-A-2
(HNDS0138-01#B CNC42(+/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi015-A-3
(HNDS0138-01#B CC38(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi015-A-4
(HNDS0138-01#B CC51(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi015-A-5
(HNDS0138-01#B CC6(-/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi015-A-6
(HNDS0138-01#B CC11(-/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi016-A-1
(HNDS0151-01#B CNC20(+/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi017-A
(HNDS0164-01#D)
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi018-A
(HNDS0176-01#A)
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi019-A
(HNDS0179-01#B)
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi014-A
(HNDS141-01#B)
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi013-A
(HNDS0143-01#A)
Donor diseases:
PTEN hamartoma tumor syndrome
CBIGi001-A-2
(PINK1-KO, PINK1-KO/AIW002-02)
CBIGi001-A-3
(PINK1-KO/PRKN-KO/AIW002-02, PINK1-KO/PRKN-KO)
BCHi015-A
(HNDS0138-01#B)
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi016-A
(HNDS0151-01#B)
Donor diseases:
PTEN hamartoma tumor syndrome
Last update 24th February 2024
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Provider

Generator Children's Hospital (BCH)

External Databases

BioSamples SAMEA115345248

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity White; American

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is affected.
Synonyms
  • PHTS
  • PTEN Hamartoma Tumor Syndrome

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

External Databases (Donor)

BioSamples SAMEA115345244

Ethics

Also have a look at the ethics information for the parental line BCHi020-A .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line BCHi020-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Medium Other medium:
Base medium: Stemflex
Main protein source: Bovine Serum Albumin (BSA)
Serum concentration: 1 %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line BCHi020-A .

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY; no clonal abnormalities
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Synonyms
  • PHTS
  • PTEN hamartoma tumor syndrome
Genetic modifications
PTEN (target)
Isogenic modification
cytoband location: 10q23
NM_000314.8: c.389 G>A, p.R130Q
Homozygous
Changed