HiPS-GLUT1DS1

General

Cell Line

hPSCreg name BRCi036-A
Cite as:
BRCi036-A
Alternative name(s)
HiPS-GLUT1DS1
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 6th June 2024
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Provider

Generator RIKEN BioResource Research Center (BRC)

External Databases

BioSamples SAMEA115659957

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male
Ethnicity Asian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Classic GLUT1 deficiency syndrome
  • Classic GLUT1-DS
  • De Vivo disease
  • Encephalopathy due to GLUT1 deficiency
show more synonyms

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46XY
Karyotyping method: Array CGH

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA115709862

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. Prof. Satoru Takahashi, Asahikawa Medical University
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Asahikawa Medical University
Approval number C21135
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Mononuclear cells collected from peripheral blood.
Synonyms
  • Peripheral Blood Mononuclear cells
  • Derived Peripheral Blood Mononuclear Cell

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Notes on reprogramming vector detection Fluorescence of EGFP

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Laminin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
Medium Other medium:
Base medium: StemFit Ak02N
Main protein source: Albumine
Serum concentration: 0 %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
Morphology pictures
GLUT1DS1_Morphology.tiff
Scale Bar: 100 µm
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Morphology
GLUT1DS1_TeratomaHE_Endoderm.tiff
A section stained with HE from teratoma contains glandular tissues. Scale bar 100 µm.
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Morphology
GLUT1DS1_TeratomaHE_Mesoderm.tiff
A section stained with HE from teratoma contains cartilage. Scale bar 100 µm.
Ectoderm
Ont Id: UBERON_0000924
Morphology
GLUT1DS1_TeratomaEctoderm.tiff
A section stained with HE from teratoma contains neural tissues. Scale bar 100 µm.

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Passage number: 8

Other Genotyping (Cell Line)